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column chromatography silica gel

column chromatography silica gel
  • Product: column chromatography silica gel
  • From: Qingdao Canaan Packaging Industry Co.,Ltd.
  • Call us: 0086-137-9329-1621
  • Email: info@canaanok.com
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column chromatography silica gel

     This product is made from premium quality silica gel. Its main characteristic is that this product can realize separation and purification by the difference in adsorption and retaining time for different ingredients in compound.
category:
1)wide pore column chromatography silica gel(classified as industrial grade wide pore column chromatography silica gel and reagent grade column chromatography silica gel according to its purity)
(2)narrow pore column chromatography silica gel
main specifications include:
180–250μm (60-80mesh)
150–180μm (80-100mesh)
125–180μm (80-120mesh)
75–150μm (100-200mesh)
45–75μm (200-300mesh)
(other specifications can be processed according to the customer’s  demand.)

olumn chromatography silica gel SG11 series (narrow pore)

Item code

Particle size

mesh

Average pore diameter

Specific surface area

Pore volumeapproximately
CP1101 180-250μm 60-80 20-30Aо >600m2/g 0.4ml/g
CP1102 150-180μm 80-100 20-30Aо >600m2/g 0.4ml/g
CP1103 125-180μm 80-120 20-30Aо >600m2/g 0.4ml/g
CP1104 75-150μm 100-200 20-30Aо >600m2/g 0.4ml/g
CP1105 45-75μm 200-300 20-30Aо >600m2/g 0.4ml/g
Column chromatography silica gel series
wide pore)
Item code

Particle size

mesh

 

Average pore diameter

Specific surface area

Pore volumeapproximately 
CP1111 180-250μm 60-100 80-100Aо 300-400m2/g 0.8ml/g
CP1113 125-180μm 80-120 80-100Aо 300-400m2/g 0.8ml/g
CP1114 75-150μm 100-200 80-100Aо 300-400m2/g 0.8ml/g
CP1115 45-75μm 200-300 80-100Aо 300-400m2/g 0.8ml/g

3) Reagent column chromatography silica gel and its application
a reagent column chromatography silica gel belongs to colloidal-state system with solid characteristics, which is composed of colloidal particles with condensate structure.  The colloidal particle is the polycondensate of hydrated silica gel (multi-silica acid), belongs to a non-crystalline-state substance. The interspace of aggregation of colloidal particle forms the inner micro interspace structure of reagent column chromatography silica gel particle. Therefore, it is a kind of premium quality adsorption material with rich fine porous structure, high specific surface area, high purity and high activity.
the main performance of reagent column chromatography silica gel——adsorption, which depends on the fine porous structure and the surface of inner pore formed in process of the raw material silica gel production. Therefore, much attention to the fine selection of the quality of raw material (wide pore granular silica gel) must be paid first. The specifications in fine selection should be controlled: adsorption capacity 80±2%,specific surface area about 360m2/g,average pore diameter is requested around 9nm(90 Angstrom).Further process shall be made on the basis of raw material selection. its main process are:crushing raw material, classifying particle size, acid treatment, washing with purified water and drying, packing and testing.
The main parameters that need controlling for reagent column chromatography:
chloride(cl)≤0.004%
iron (Fe) ≤0.02%
PH(10% water suspension)5-6
the main uses of reagent column chromatography silica gel as follows:
(1) used for Chinese herb medicine and chemical synthesis pharmaceuticals and the separation & purification of biological active substance;
(2) used for abstraction of such polypeptide of macromolecule protein as biological active substance in industrial process of biological ferment and also used for acid engineering technology;
(3) preparation of highly-purified substances through separation & adsorption of column chromatography silica gel;
(4) refining & dehydration of organic substance;
(5) removal of harmful ingredients from edible vegetable oil;
(6) used for refining of petroleum articles, such as extracted oil ( the retained part of naphtha at 600C-900C), removal of the impurity like aromatic hydrocarbon.

    The mechanism about separation, purification and dehydration for column chromatography silica gel: there is no much difference between the microcosmic structure of column chromatography silica gel and the general silica gel.SiO2 forming colloidal skeleton is combined in silica tetrahedron and the force field between atoms is in equilibrium. As stated above, silica gel has very high specific surface area,the surface structure of the interspace inside silica gel particle is different from the inner structure of the formed skeleton, the surface silicon atom and the constitutional water contained inside the colloidal gel form silanophilic (Si—OH), i.e. the unbalancedness of this structure causes the surface of silica gel to create free force field, which has adsorption capacity to water molecule or other polar molecule, the surface of colloidal particle has different adsorption capacity on the adsorbed substance due to different strength of polar molecule the adsorbed substance. Because of this regard, silica gel has the selective adsorption on different compound substance. When the component with stronger polar passes through the surface of silica gel, the adsorption strength created with silica gel is also stronger and this component retains on the surface of silica gel for longer time;The other way round, for the component with weaker polar, it retains for shorter time. Therefore, the compound with different substances can be separated due to the different retaining time in process of passing through silica gel. For the strong polar substance, silica gel has strong adsorption capacity on it, such as water molecule. In this case, the molecule of the adsorbed substance can break away from the surface of silica gel by overcoming the potential barrier of gravitational field created on the surface of silica gel only when obtaining enough energy ( for example, heat energy). Thus, at normal conditions, strong polar component is kept inside the interspace of silica gel when compound with strong polar components passes through silica gel column chromatography, which reflects silica gel’s capacity for dehydration & refining or purification of substance.
The prominent advantages of column chromatography silica gel in its application in biology engineering technology
(1)with rigid skeleton structure, high mechanical strength,can stand the pressure within 30MPa;
(2)excellent adsorption performance; It has ideal separation function to similar character and structure, even to isomer.
(3)it has good thermal stability and chemical stability;
(4)compared with organic column padding, silica gel is a solid, colloidal gel based on SiO2 matrix,its structure is compact, which will not pollute target product because there is no organic matter that can flow in process of application;
(5)It can have selective adsorption and purification of the components of isomer from multi-component solution;
(6)in preparation of column chromatography silica gel, a series of products with average pore diameter 20Å-20000Å can be produced through the control of different process conditions in order to adapt to the separation & purification of the substances with different character, molecular weight and molecular structure.

packing:25 KG/composite bag,or 25KG/cardboard drum
note:We can process the product according to your demand if you have any special request.